GRSDB allows the user to analyze the transcribed regions of eukaryotic pre-mRNA sequences with respect to information about the composition of Quadruplex forming G Rich Sequences (QGRS) and their location relative to the RNA processing sites. These putative G-quadruplexes are identified using the following motif.
Here x = # guanine tetrads in the G-quadruplex and y1, y2, y3 = length of gaps (that is, the length of the loops connecting the guanine tetrads). So the motif consists of four equal length groups of guanines, separated by arbitrary nucleotide sequences, with the following restrictions:
x=2, y1=2, y2=0, y3=3|
x=3, y1=1, y2=10, y3=2
x=4, y1=1, y2=1, y3=1
The first sequence has four tetrads and equal length gaps. This would seem to provide the most stable G-quadruplex. The second sequence is notable for the significant differences in the size of its loops. The third sequence has two tetrads, even though three of the G-groups could have included another G (since all G-groups must be equal in size).
There are two categories of QGRS that are referred to in GRSDB: